Structure - Activities
Consortium generated constructs are presented here alphabetically by the name of the protein or portion thereof that they produce. Selecting the construct name will take you to a second table where details of this construct and its availability are listed.
CMC Expression Constructs
| Target | Amino Acids | Portion Produced | Construct Name | Tag / Cleavable | Comments | Protocol | Contact (Collaborators) |
|---|---|---|---|---|---|---|---|
| Alpha Actinin | 1-892 | Alpha actinin - full length | pET15b-aActinin | His/thrombin | soluble | protocol | Liddington |
| Alpha Actinin | 269-444 | Alpha actinin - spectrin repeats | pET15b-aActinin-SR | His/thrombin | soluble | Liddington | |
| Alpha Catenin | 1-906 | Alpha catenin -full length | pBS-aCatenin | no tag | shuttle vector | Liddington | |
| Alpha Catenin | 277-510 | Alpha catenin -domain 3 | pET15b-CD3 | His/thrombin | soluble | Liddington | |
| DOCK180 | 154-256 | DOCK180 DHR1 domain | pGEX-6P2 | ? | protocol | Liddington | |
| FAK | 1-1052 | FAK-full length | FAK-baculovirus | His/non-cleavable | soluble expression | Liddington (Viori) | |
| FAK | 1-1052 | FAK-full length | pT7-7-FAK | no tag | protocol | Liddington (Viori) | |
| GIT1 | 1-770 | GIT1 -full length | GIT1-baculovirus | N-ThioHis/enterkinase | soluble expression | protocol | Liddington |
| GIT1 | 624-770 | GIT1 -paxillin binding domain | pGEX4T3-GIT1c | GST/thrombin | soluble expression | protocol | Liddington (Horwitz) |
| Layilin / Talin | 207-400 | Layilin tail+talin F2+F3 fusion protein | pET15b-layilin | His/thrombin | Liddington | ||
| Leupaxin | 1-386 | Leupaxin -full length | pET15b-leupaxin | His/HA, His/thrombincleavable, HA not | Ginsberg | ||
| MEK1 | ? | Full-length MEK1 | pRSETA-MEKK1 | His non-cleavable | Liddington (Yian) | ||
| Paxillin | 1-557 | Paxillin -full length | pET28-paxillin | His/thrombin | mut in Histag,protein not in frame with start | Liddington (Schwarzenbacher) | |
| Paxillin | 38-557 | Paxillin (N-terminal truncated) | pGEX4T2-paxillin 38-557 | GST/FLAG, GST-TEVcleavable FLAG not | not soluble poor yield | Liddington (Vogel) | |
| Plectin | 59-293 | Plectin - Actin binding domain (ABD) | pET15b-plectin6 | His/thrombin | soluble/great yield | protocol | Liddington (Pereda) |
| Plectin | 59-293 | Plectin -ABD domain mutant | pET15b-plectin6ss-mut | His/thrombin | soluble/great yield | ||
| Talin | 1-400 | Talin Head domain | pET15b-His-Talin Head | His/thrombin | soluble/ok yield | protocol | Liddington (Pereda) |
| Talin | 196-400 | Talin FERM domain (F2F3 portion) | pET15b-His-F2F3 | His/thrombin | soluble/great yield | protocol | Liddington (Pereda) |
| Talin / PIPKIg | Talin Fragment G (L2094A/I12095A mutant) | pET-29+ | |||||
| Talin / PIPKIg | 1-2541 | Talin-full length, S429D gain-of-function mutant | pET30a-talin | His/non-cleavable | soluble | Liddington (Zhang) | |
| Talin / PIPKIg | 1-2541 | Full length Talin | pET30a-talin | His/non-cleavable | soluble | protocol | Liddington (Zhang) |
| Talin / PIPKIg | 1-2541 | Talin-full length, S446D gain-of-function mutant | pET30a-talin | His/non-cleavable | soluble | Liddington (Zhang) | |
| Talin / PIPKIg | 1-2541 | Talin-full length, S429D/S446D double gain-of-function mutant | pET30a-talin | His/non-cleavable | soluble | Liddington (Zhang) | |
| Talin / PIPKIg | 1647-2541 | Talin C-terminal fragment | pET15b-talin 1647-2541 | His/thrombin | soluble/great yield | protocol | Liddington (Critchley) |
| Talin / PIPKIg | 1843-1973 | Talin VBS2 | pET151-DTOPO talin 1843-1973 | His/TEV | soluble/good yield | protocol | Liddington (Critchley) |
| Talin / PIPKIg | 196-889 | Talin FERM F2F3 + part of rod | pET151-DTOPO talin 196-889 | protocol | |||
| Talin / PIPKIg | 1984-2344 | Talin VBS3 | pGEX-4T-2 | ||||
| Talin / PIPKIg | 2071-2135 | Talin's actin-binding domain | pGEX-4T-2 | ||||
| Talin / PIPKIg | 209-400 | Talin F2F3 fused to PIPKIgamma tail | pET15b-His-F2F3short | His/thrombin | soluble/great yield | Liddington (Pereda) | |
| Talin / PIPKIg | 309-405 | Talin FERM F3 portion | pET15b-His-F3 | His/thrombin | low yield | protocol | Liddington (Pereda) |
| Talin / PIPKIg | 309-405 | Talin FERM F3 portion | pMAL-cgx-F3,4 | MBP-6His/FactorXa | soluble/ok yield | Liddington | |
| Talin / PIPKIg | 397-2541 | Talin rod | pET30b-talin rod | N-His Calpain | protocol | Liddington (Lam) | |
| Talin / PIPKIg | 482-636 | Talin's Vinculin-binding domain (VBS1; 4-helix bundle) | pET15b-talin 482-636 | His/thrombin | soluble/great yield | protocol | Liddington (Critchley) |
| Talin / PIPKIg | 482-655 | Talin's VBS1 (autoinhibited 5-helix bundle) | pET15b-talin 482-655 | His/thrombin | soluble/great yield | protocol | Liddington (Critchley) |
| Talin / PIPKIg | 482-789 | Talin rod fragment | pET15b-talin 482-789 | His/thrombin | soluble/great yield | protocol | Liddington (Critchley) |
| Talin / PIPKIg | 482-889 | Talin rod fragment | pET151-DTOPO talin 482-889 | His/TEV | soluble/great yield | protocol | Liddington (Critchley) |
| Talin / PIPKIg | 482-889 | Talin 482-889 stabilized fragment quadruple mutant (T775V/T809I/T833I/T867V) | pET151-DTOPO talin 482-889TTTT | His/TEV | soluble/great yield | protocol | Liddington (Critchley) |
| Talin / PIPKIg | 755-889 | Talin's Vinculin-binding domain 2 (VBS2) | pET15b-talin 755-889 | His/thrombin | soluble/great yield | protocol | Liddington (Critchley) |
| Tensin | 1514-1631 | Tensin SH2 domain | pET15b-SH2 | protocol | Hanein (Lin) | ||
| Tensin | 1514-1631 | Tensin SH2+PTB domains | pET15b-SH2-PTB | protocol | Hanein (Lin) | ||
| Tensin | 1653-1786 | Tensin PTB domain | pET15b-PTB | protocol | Hanein (Lin) | ||
| Vinculin | 1-258 | Vinculin Head domain (Y100E mutant) | pET15b-VD1 | protocol | Liddington | ||
| Vinculin | 1-258 | Vinculin -head domain 1 | pET15b-VD1 | Liddington | |||
| Vinculin | 879-1066 | Vinculin Tail (Y1064E mutant) | pET15b-VD1 | protocol | Liddington (Bakolista) |