| Phosphoproteomics |
Phosphorylation of FLAG-GFP-PAXILLIN
CMCKB: FLAG-GFP-Paxillin (PXN, chicken)
| C18 (+) inhibitors | Alignment / Methods |
| IMAC (+) inhibitors | Alignment / Methods |
| Site | Position | Peptide | ||
|---|---|---|---|---|
| (+) Inhib | (-) Inhib | |||
| 31Y | 21 - 32 | RPVFLTEETPYS | ||
| 31Y | 21 - 37 | RPVFLTEETPYSYPTGN | + | |
| 31Y | 21 - 44 | RPVFLTEETPYSYPTGNHTYQEIA | +++ | |
| 83S / 85S / 88Y | 76 - 93 | YGHQQPQSQSPIYSSSAK | ||
| 83S / 88Y | 76 - 93 | YGHQQPQSQSPIYSSSAK | ||
| 85S | 76 - 93 | YGHQQPQSQSPIYSSSAK | ++++ | +++ |
| 85S / 88Y | 76 - 93 | YGHQQPQSQSPIYSSSAK | +++ | |
| 85S / 88Y / 89S | 76 - 93 | YGHQQPQSQSPIYSSSAK | ||
| 88Y | 76 - 93 | YGHQQPQSQSPIYSSSAK | ++++ | |
| 94S-95S / 98S | 94 - 101 | [SS]SASVPR | ||
| 94S-96S / 98S / 106S | 94 - 110 | [SSS]ASVPRDGLSSPSPR | ||
| 98S | 94 - 101 | SSSASVPR | + | |
| 106S | 102 - 110 | DGLSSPSPR | ++++ | +++ |
| 106S / 108S | 102 - 110 | DGLSSPSPR | +++ | |
| 108S | 102 - 110 | DGLSSPSPR | +++ | +++ |
| 112S / 119S | 111 - 123 | ASEEEHVYSFPNK | ||
| 118Y | 111 - 123 | ASEEEHVYSFPNK | ++++ | |
| 118Y | 112 - 123 | SEEEHVYSFPNK | ||
| 118Y / 119S | 111 - 125 | ASEEEHVYSFPNKQK | ||
| 126S | 124 - 147 | QKSAEPSPTMTSTSLGSNLSELDR | +++ | +++ |
| 126S / 130S | 124 - 147 | QKSAEPSPTMTSTSLGSNLSELDR | ++++ | +++ |
| 126S / 130S | 126 - 147 | SAEPSPTMTSTSLGSNLSELDR | ++ | |
| 126S / 130S / 137S | 124 - 147 | QKSAEPSPTMTSTSLGSNLSELDR | ||
| 130S | 126 - 147 | SAEPSPTMTSTSLGSNLSELDR | ++ | +++ |
| 137S | 126 - 147 | SAEPSPTMTSTSLGSNLSELDR | ++ | |
| 164S | 148 - 170 | LLLELNAVQHNPPSGFSADEVSR | ++ | |
| 171S | 171 - 194 | SPSLPNVTGPHYVIPESSSSAGGK | ++++ | +++ |
| 171S / 182Y | 171 - 194 | SPSLPNVTGPHYVIPESSSSAGGK | +++ | |
| 171S / 187S-190S | 171 - 194 | SPSLPNVTGPHYVIPE[SSSS]AGGK | ||
| 173S | 171 - 194 | SPSLPNVTGPHYVIPESSSSAGGK | ++++ | +++ |
| 173S / 182Y | 171 - 194 | SPSLPNVTGPHYVIPESSSSAGGK | ++ | |
| 182Y | 171 - 194 | SPSLPNVTGPHYVIPESSSSAGGK | ++++ | |
| 227S-228S / 231S | 210 - 248 | GIEDVRPSVESLLDELE[SS]VPSPVPAITVSQGEVSSPQR | ||
| 227S-228S / 231S / 245S | 210 - 248 | GIEDVRPSVESLLDELE[SS]VPSPVPAITVSQGEVSSPQR | ||
| 231S | 210 - 248 | GIEDVRPSVESLLDELESSVPSPVPAITVSQGEVSSPQR | ++++ | |
| 231S / 245S | 210 - 248 | GIEDVRPSVESLLDELESSVPSPVPAITVSQGEVSSPQR | +++ | |
| 239S / 245S | 210 - 248 | GIEDVRPSVESLLDELESSVPSPVPAITVSQGEVSSPQR | ||
| 245S | 210 - 248 | GIEDVRPSVESLLDELESSVPSPVPAITVSQGEVSSPQR | ++++ | |
| 245S | 220 - 248 | SLLDELESSVPSPVPAITVSQGEVSSPQR | + | |
| 259S | 258 - 265 | ISASSATR | +++ | |
| 290S | 285 - 297 | AGGSSSPPSTTPK | +++ | +++ |
| 290S | 285 - 315 | AGGSSSPPSTTPKPGSQLDTMLGSLQSDLNK | ++++ | ++++ |
| 382S | 376 - 384 | DYHNLFSPR | ||
| 501S | 499 - 513 | RGSLCSGCQKPITGR | +++ | +++ |
*Peptides detected only by
enrichment with
immobilized metal-affinity chromatography (IMAC).
# Peptides which do not require enrichment by IMAC for detection.