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Genetics / Screens - Approaches

Genes Implicated in Border Cell Migration

Gene Protein Product Biochemical activity Proposed role in migration Publications
slbo C/EBP Transcriptional activator Required for initiation of migration Montell et al. 1992, Rorth and Montell, 1992, Rorth et al., 2000
chic Profilin Actin monomer binding protein stimulates actin polymerization Regulates G-actin pool Verheyen and Cooley, 1994
RacI Rac 21kD GTPase Actin polymerization at leading edge Murphy and Montell, 1996
shg E-cadherin Homophilic cell adhesion Border cell-border cell adhesion and border cell- nurse cell traction Niewiadomska et al., 1999, Oda et al., 1997
zip Myosin II Barbed end directed actin motor Retraction of trailing edge Edwards and Kiehart, 1996
jing AEBP2 homolog Zn finger Transcriptional activator May cooperate with C/EBP Liu and Montell, 2001
tai AIB1 homolog Steroid hormone receptor coactivator Regulates timing of migration in response to ecdysone Bai et al., 2000
EcR EcR Hormone binding subunit of ecdysone receptor Same as TAI Bai et al., 2000
usp RXR homolog Ecdysone receptor subunit Same as TAI Bai et al., 2000
ecd Not known Ecdysone biosynthesis Regulates ecdysone production Bai et al., 2000
Pvf1 PVF1 or VEGF17E Secreted ligand for receptor tyrosine kinase Guidance of border cells towards the oocyte. May function redundantly with GRK, PVF2 or PVF3 Duchek et al., 2001, McDonald et al., 2003
pvr PVR Receptor for PVF1, PVF2 and PVF3 Guidance of border cells to the oocyte. May function redundantly with EGFR Duchek et al., 2001, McDonald et al., 2003
Egfr* EGFR EGF receptor homolog Functions redundantly with PVR to guide border cells to the oocyte and guides border cells to the dorsal side, once they reach the oocyte Duchek et al., 2001, Duchek and Rorth, 2001
mbc Dock180 Rac exchange factor Actin dynamics Duchek et al., 2001
Stat92E STAT Signal activated transcription factor Functions autonomously within border cells to cause cells to become migratory in response to signal from polar cells Silver and Montell, 2001, Beccari et al., 2002, Xi et al., 2003, Silver et al., 2005
os UPD Secreted cytokine that activates the JAK/STAT pathway Paracrine signal that stimulates cells next to the polar cells to cluster around the polar cells and migrate. Silver and Montell, 2001
hop Janus kinase homolog Non receptor tyrosine kinase Its activation is necessary and sufficient to convert epithelial follicle cells to migratory cells Silver and Montell, 2001
dome DOME UPD receptor It is required for JAK/STAT activity Silver and Montell, 2001
jar Myosin VI Pointed end directed actin motor Required to stabilize E-cadherin and maybe for protrusion of actin filaments Geisbrecht and Montell, 2002
tsr Cofilin Actin depolymerizing factor Regulates actin filament dynamics Chen et al., 2001
cher Filamin Actin bundling factor Regulates actin organization Sokol and Cooley, 2003
th DIAP1 Caspase inhibitor Suppresses migration defects due to dominant-negative Rac Geisbrecht and Montell, 2004
mrtf MAL-D Transcriptional cofactor for SRF Mediates transcriptional response to tension and regulation of the actin cytoskeleton Somogyi and Rorth, 2004
bs SRF Transcription factor Same as MAL-D Somogyi and Rorth, 2004
baz BAZ or PAR-3 Epithelial polarity protein that interacts with PAR-6 and atypical protein kinase C (aPKC) Required to maintain proper distributions of membrane receptors such as PVR and E-cadherin Pinheiro and Montell, 2004, Abdelilah-Seyfried et al., 2003
par-6 PAR-6 Epithelial polarity protein that interacts with PAR-3 and aPKC Required to maintain proper distributions of membrane receptors such as PVR and E-cadherin Pinheiro and Montell, 2004
shi Dynamin Large GTPase required for endocytosis Required for STAT nuclear translocation and Localization of phosphotyrosine Silver et al., 2005, Medioni and Noselli, 2005, Jekely et al., 2005
Rab5 Rab5 Small GTPase Involved in endocytosis Medioni and Noselli, 2005
Cbl E3 ubiquitin ligase Attaches ubiquitin to target proteins Targets activated PVR and EGFR for directed recycling to leading edge Jekely et al., 2005
spri* Sprint Ras interacting protein with SH2 and Ysp9 domains Functions redundantly with Cbl to concentrate active RTK signaling at leading edge Jekely et al., 2005
aop Yan ETS family transcriptional repressor Promotes endocytosis and downregulation of E-cadherin Schober et al., 2005
N Notch Transmembrane receptor/ transcription factor Unknown Schober et al., 2005, Gonzalez-Reyes and St Johnston, 1998
Cortactin Cortactin Src substrate, cortical actin binding protein Possible minor role in actin dynamics Somogyi and Rorth, 2004
* mutation causes no phenotype unless combined with another mutations null mutation shows a mild phenotype        
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